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Plant RelA/SpoT Homolog Confers Salt Tolerance in Escherichia coli and Saccharomyces cerevisiae
https://saga-u.repo.nii.ac.jp/records/13442
https://saga-u.repo.nii.ac.jp/records/1344227cb3bb5-4f58-42b5-93a2-47a77ce8034c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-02-28 | |||||
| タイトル | ||||||
| タイトル | Plant RelA/SpoT Homolog Confers Salt Tolerance in Escherichia coli and Saccharomyces cerevisiae | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | salt tolerance, Suaeda japonica | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamada, A
× Yamada, A× Tsutsumi, K× Tanimoto, S× Ozeki, Y |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | To analyze the mechanism of salt tolerance in the halophyte Suaeda japonica, Escherichia coli was used as a host organism to undertake functional screening of cDNAs encoding proteins that may play an important role for the salt-tolerance mechanisms. A transformant expressing RelA/SpoT homolog (Sj-RSH) was found to have enhanced salt tolerance. In E. coli, RelA/SpoT controlled the amount of guanosine tetraphosphate (ppGpp) and guanosin pentaphosphate (pppGpp), which are the effectors of the bacterial stringent response. Complementation analysis using the relA mutant of E. coli showed that Sj-RSH conferred the phenotype associated with (p)ppGpp synthesis. Furthermore, expression of Sj-RSH driven bt the GAL1 promoter also gave rise to enhanced salt tolerance in yeast. Northern blot analysis of the yeast transformant revealed that the transcriptional levels of stress responsive genes including GPD1, VMA1, BMH1, HYP1 and HOG1 were clearly enhanced in the Sj-RSH transformant when compared with an empty vector transformant under stress-free and 1.5 M NaCl stress conditions. These results suggested that (p)ppGpp synthesis mediated by plant RelA/SpoT homologs plays a critical role for the transcriptional induction of several stress responsive genes, directly or indirectly in yeast, and that the conserved stress-resistance system may exist in higher plants. | |||||
| 書誌情報 |
PLANT AND CELL PHYSIOLOGY 巻 44, 号 1, p. 3-9, 発行日 2003 |
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| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 雑誌掲載論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Journal Article | |||||
| 資源タイプ・DCMI | ||||||
| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 01 | |||||
